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KMID : 0352720180420020175
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Journal of Ginseng Research
2018 Volume.42 No. 2 p.175 ~ p.182
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Effect of azoxystrobin fungicide on the physiological and biochemical indices and ginsenoside contents of ginseng leaves
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Liang Shuang
Xu Xuanwei
Lu Zhongbin
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Abstract
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Background: The impact of fungicide azoxystrobin, applied as foliar spray, on the physiological and biochemical indices and ginsenoside contents of ginseng was studied in ginseng (Panax ginseng Mey. cv. “Ermaya”) under natural environmental conditions. Different concentrations of 25% azoxystrobin SC (150 g a.i./ha and 225 g a.i./ha) on ginseng plants were sprayed three times, and the changes in physiological and biochemical indices and ginsenoside contents of ginseng leaves were tested.
Methods: Physiological and biochemical indices were measured using a spectrophotometer (Shimadzu UV-2450). Every index was determined three times per replication. Extracts of ginsenosides were analyzed by HPLC (Shimadzu LC20-AB) utilizing a GL-Wondasil C18 column.
Results: Chlorophyll and soluble protein contents were significantly (p=0.05) increased compared with the control by the application of azoxystrobin. Additionally, activities of superoxide dismutase, catalase, ascorbate peroxidase, peroxidase, and ginsenoside contents in azoxystrobin-treated plants were improved, and malondialdehyde content and O2? contents were reduced effectively. Azoxystrobin treatments to ginseng plants at all growth stages suggested that the azoxystrobin-induced delay of senescence was due to an enhanced antioxidant enzyme activity protecting the plants from harmful active oxygen species. When the dose of azoxystrobin was 225 g a.i./ha, the effect was more significant.
Conclusion: This work suggested that azoxystrobin played a role in delaying senescence by changing physiological and biochemical indices and improving ginsenoside contents in ginseng leaves.
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KEYWORD
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azoxystrobin, ginsenoside contents, leaves, Panax ginseng, physiological and biochemical indices
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